SelectCore 96-well plates contain a portfolio of monodispersed PVP/DVB polymer sorbents, which have good aqueous wettability, excellent chemical stability, and high capacity for most acidic, basic, and neutral compounds. They are widely used in catecholamines and metanephrines, hormones, fat-soluble vitamins and other clinical analysis. They are ideal for rapid sample clean-up and analyte enrichment with high throughput and recovery.

Main Features

• Advanced monodispersed particle technology for high separation efficiency 

• Wide pH range (pH1-14)

• High capacity and good recovery

• Good batch-to-batch consistency

Specifications

Applications

Clinical Analysis of Plasma Catecholamines and Metanephrines by HPLC-MS/MS

Clinical analysis of catecholamines and metanephrines extracted from plasma with SelectCore WCX 96-well plate.

（1）Condition SelectCore WCX 96-well plate with 200 µL of MeOH and 200 µL of water,

（2）Dilute the pooled plasma samples (200 µL) with 200 µL of 50 mM ammonium acetate and then spike with 50 µL of internal standard solution,

（3）Load the pre-treated samples (350 µL) in individual wells of SelectCore WCX 96-well plate,

（4）Wash the 96-well plate with 200 µL of 25 mM ammonium acetate followed by 200 µL of MeCN, and then dry it under vacuum for 30 s,

（5）Elute the target catecholamines compounds from the plate with 70 µL aliquots of 85:15 MeCN:H₂O containing 2% formic acid into 96-well sample collection plate,

（6）Evaporate the collected samples to dryness and reconstituted them in 40 μL of 97:2:1 H₂O:MeCN:FA solution.