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BioCore SEC Columns

​BioCore SEC is a family of high performance, size exclusion chromatography columns, designed for separating antibodies, proteins, peptides, oligonucleotides and related substances. The column technology involves creation of an inert neutral hydrophilic layer onto high strength, high pore-volume, monodispersed porous silica particles, combined with well-established column packing processes. BioCore SEC columns have a broad application range in (bio)pharmaceutical, biotechnology and academic research.
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BioCore SEC is a family of high performance, size exclusion chromatography columns, designed for separating antibodies, proteins, peptides, oligonucleotides and related substances. The column technology involves creation of an inert neutral hydrophilic layer onto high strength, high pore-volume, monodispersed porous silica particles, combined with well-established column packing processes. BioCore SEC columns have a broad application range in (bio)pharmaceutical, biotechnology and academic research.

Main Features

  • High column efficiency and high resolution

  • Minimal undesired interactions between the stationary phase and analytes, resulting in good peak shape and recovery

  • High mechanical strength for longer column lifetime

  • Broad application range, including small-molecule drugs, peptides, proteins, oligos, glycans, etc.

Specifications

Product Name

SEC-120

SEC-150

SEC-300

SEC-500

Functional Group

Diol

Substrate

Monodispersed, high pore volume, porous, spherical, silica particles

Particle Size

1.8, 3 & 5 μm

Pore Size

120 Å

150 Å

300 Å

450 Å

Pressure Limit

6000 psi for 1.8 µm

3000 psi for 3 µm

1500 psi for 5 µm

Temperature Limit

40 ℃

pH Range

2-8

Calibration Curve（PEG）

NA

500-15000

1000-50000

5000-200000

Calibration Curve（Glucan）

NA

1000-30000

2000-100000

20000-500000

Calibration Curve（Globular protein）

NA

5000-150000

10000-750000

20000-1500000

Application

Small-molecule drugs, peptides, glycans, small oligos

Small-molecule drugs, peptides, glycans, small oligos and small proteins

mAbs and aggregates

mAbs and high order aggregates, large proteins and large DNA/RNA

Calibration Curve

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Applications

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Product Name

Particle Size

(µm)

Length

(mm)

ID (mm)

7.8

4.6

BioCore SEC-120

5

300

B213-050012-07830S

B213-050012-04630S

150

B213-050012-07815S

B213-050012-04615S

50

/

B213-050012-04605S

3

300

B213-030012-07830S

B213-030012-04630S

150

B213-030012-07815S

B213-030012-04615S

50

/

B213-030012-04605S

1.8

300

/

B213-018012-04630S

150

/

B213-018012-04615S

50

/

B213-018012-04605S

BioCore SEC-150

5

300

B213-050015-07830S

B213-050015-04630S

150

B213-050015-07815S

B213-050015-04615S

50

/

B213-050015-04605S

3

300

B213-030015-07830S

B213-030015-04630S

150

B213-030015-07815S

B213-030015-04615S

50

/

B213-030015-04605S

1.8

300

/

B213-018015-04630S

150

/

B213-018015-04615S

50

/

B213-018015-04605S

BioCore SEC-300

5

300

B213-050030-07830S

B213-050030-04630S

150

B213-050030-07815S

B213-050030-04615S

50

/

B213-050030-04605S

3

300

B213-030030-07830S

B213-030030-04630S

150

B213-030030-07815S

B213-030030-04615S

50

/

B213-030030-04605S

1.8

300

/

B213-018030-04630S

150

/

B213-018030-04615S

50

/

B213-018030-04605S

BioCore SEC-500

5

300

B213-050050-07830S-V

B213-050050-04630S-V

150

B213-050050-07815S-V

B213-050050-04615S-V

50

/

B213-050050-04605S-V

3

300

B213-030050-07830S

B213-030050-04630S

150

B213-030050-07815S

B213-030050-04615S

50

/

B213-030050-04605S

1.8

300

/

B213-018050-04630S

150

/

B213-018050-04615S

50

/

B213-018050-04605S

BioCore SEC-1000

5

300

B213-050100-07830S

B213-050100-04630S

150

B213-050100-07815S

B213-050100-04615S

50

/

B213-050100-04605S


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