DNACore AAV-SEC is a family of high performance, size exclusion chromatography columns, designed for aggregates analysis of AAVs in different serotypes.

Main Features

• Innovative particle technology: monodispersity for high efficiency, high mechanical strength for long column lifetime, and large pore-volume for high resolution

• Advanced column chemistry for minimal undesired secondary interactions

• Robust column packing for good column lifetime

• Good column-to-column consistency

Specifications

Applications

AAV Aggregates Analysis in Different Serotypes

High Throughput Analysis

Using a 150 mm DNACore AAV-SEC column, both monomer and aggregates of four AAV serotypes were well separated (Rs > 2.0) under the same conditions. This shortened analysis time by half and doubled throughput compared to a 300mm column.

Batch-to-Batch Reproducibility

Three different batches of DNACore AAV-SEC columns were tested using AAV2. The chromatograms showed consistent monomer peak times, monomer ratios, and resolutions (RSD < 1%) among the three batches, indicating good batch-to-batch consistency of DNACore AAV-SEC columns.